Source. 



.Date 



of Isolation Name. 



. Group No. (1) . 



Agar 

 Hanging-block 



DETAILED FEATURES 

 NOTE. — Underscore required terms. Observe notes 

 and glossary of terms on opposite side of card. 

 I. MORPHOLOGY(!) 



1. Vegetative Cells, iVIedium used • 



temp age days 



Form, round, short rods, long rods, short chains, 

 Ions chains, filaments, commas, short spirals, long 

 spirals, Clostridium, cuneate, clavate, curved. 



Limits of Size 



Size of Majority 



Ends, rounded, truncate, concave. 



I Orientation (grouping) 



Chains (No. of elements) .... 

 Agar I ^f,gfi chains, long chains 



angmg-block Orientation of Chains, parallel 



[ irregular. 



2. Sporangia, medium used , temp 



age days 



Form, elliptical, short rods, spindled, clavate, drum- 

 t sticks. . ,. , , . ., 



Limits of Size Size of Majority 



f Orientation (grouping) 



I Chains (No. of elements) .... 

 ] Orientation of Chains, parallel, 

 \ irregular. 

 Location of Endospores, central, polar. 



3. Endospores. 



Form, round, elliptical, elongated. 



Limits of Size. , 



Size of Majority ' . 



Wall, thick, thin. 



Sporangium wall, adherent, not adherent. 

 Germination, equatorial, oblique, polor, bipolar, by 

 stretching. 



4. Flagella No Attachment polar, bipolar, per- 



itrichiate. How Stained 



5. Capsules, present on 



6. Zoogloea, Pseudozoogloea. 



7. Involution Forms, on in days at C. 



8. Staining Reactions. , , t i. ■ 

 1:10 watery fuchsin, gentian violet, carbol tuchsm, 



Loffler's alkaline methylene blue. 

 Special Stains 



Gram Glycogen 



Fat Acid fast 



Neisser 



II. CULTURAL FEATURES (s) 



1. Agar Stroke. 



Growth, invisible, scanty, moderate, abundant. 



Form of growth, filiform, echinulate, beaded, spreading 



plumose, arborescent, rhizoid. 

 Elevation of growth, flat, effuse, raised, convex. 

 Luster, glistening, dull, cretaceous. 

 Topography, smooth, contoured, rugose, verrucose. 

 Optical Characters, opaque, translucent, opalescent, 



iridescent. 



Chromogenesis (*) 



Odor, absent, decided, resembling 



Consistency, slimy, butyrous. viscid, membranous, 



coriaceous, brittle. 

 Medium grayed, browned, reddened, blued, greened. 



2. Potato. 



Growth, scanty, moderate, abundant, transient, per- 

 sistent. 



Form of growth, filiform, echinulate, beaded, spreading, 

 plumose, arborescent, rhizoid. 



Elevation of growth, flat, effuse, raised convex. 



Luster, glistening, dull, cretaceous. 



Topography, smooth, contoured, rugose, verrucose. 



Chromogenesis (») Pigment in water 



insoluble, soluble; other solvents 



Odor, absent, decided, resembling 



Consistency, slimy, butyrous, viscid, membranous, 

 coriaceous, brittle. 



Medium grayed, browned, reddened, blued, greened. 



3. LbSler's Blood Serum. 



Stroke, invisible, scanty, moderate, abundant. Form 

 of growth, filiform, echinulate, beaded, spreading, 

 plumose, arborescent, rhizoid. 



Elevation of growth fiat, effuse, raised, convex. 



Luster, glistening, dull, cretaceous. 



Topography, smooth, contoured, rugose, verrucose. 



Chromogenesis {") 



Medium grayed, browned, reddened, blued, greened. 



Liquefaction begins in d., complete in d. 



4. Agar Stab. 



Growth uniform, best at top, best at bottom; surface 

 growth scanty, abundant; restricted, wide-spread. 



Line of puncture, filiform, beaded, papillate, villous, 

 plumose, arborescent; liquefaction. 



5. Gelatin Stab. 1^,1.. 

 Growth uniform, best at top, best at bottom. 



Line of puncture, filiform, beaded, paptllate, villous, 

 plumose, arborescent. . ,., ,. 



Liquefaction, crateriform, napiform, mfundibuli- 



form, saccate, startiform; begins in ". 



complete in d. 



6. Nutrient Broth. , „ , t , 

 Surface growth, ring, pellicle, flocculent, membranous. 



Clouding slight, moderate, strong; transient, persistent; 



none; fluid turbid. 



Odor, absent, decided, resembling . 



Sediment, compact, flocculent, granular, flaky, viscta 



on agitation, abundant, scant. 



7. Milk. 



Clearing without coagulation. 

 Coagulation prompt, delayed, absent. 



Extrusion of whey begins in . . . .days. 



Coagulum slowly peptonized, rapidly peptonizea. 



Peptonization begins on d, complete on ..... d. 



Reaction, id 2d 4d.... lod 20d.... 



Consistency, slimy, viscid, unchanged. 

 Medium browned, reddened, blued, greened. 

 Lab ferment, present, absent. 



8. Litmus Milk. 



Acid, alkaline, acid then alkaline, no change. 

 Prompt reduction, no reduction, partial slow re- 

 duction. 



9. Gelatin Colonies. 



Growth, slow, rapid. . -j „., 



Form, puncliform, round, irregular, ameboid, my- 



celioid, filamentous, rhizoid. . 



Elevation, flat, effuse, raised, convex, pulvmale, 



crateriform (.liquefying). , r , ■ ,. 



Edge, entire, undulate, lobate, erase, lacerate, fimbriate, 



filamentous, floccose, curled. 



10. Agar Colonies. . 



jGrowth slow, rapid (temperature ■)■ 



Form, puncliform, round, irregular, ameboid, my- 

 celioid, filamentous, rhizoid. . 



Surface smooth, rough, concentrically ringed, radiate, 

 striate. . , ^ 7 . , 



Elevation, flat, effuse, raised, convex, pulvmate, 

 umbonate. , , j, 1^ • , 



Edge, entire, undulate, lobate, erose, lacerate, fimbriate, 

 floccose, curled. . „ , 7 



Internal structure, amorphous, finely-, coarsely- 

 granular, grumose, filamentous, floccose, curled. 



11. Starch Jelly. 

 Growth, scanty, copious. 



Diastasic action, absent, feeble, profound. 

 Medium stained 



12. Silicate Jelly (Fermi's Solution.) 

 Growth copious, scanty, absent. 

 Medium stained 



13. Cohn's Solution. 



Growth copious, scanty, absent. 

 Medium fluorescent, non-fluorescent. 



14. Uschinsky's Solution. 

 Growth copious, scanty, absent. 

 Fluid viscid, not viscid. 



15. Sodium Chloride in Bouillon, 



Per cent inhibiting growth 



16. Growth in Bouillon over Chloroform, unrestrained, 



feeble, absent. 



17. Nitrogen. Obtained from peptone, asparagin, glyco- 



coll, urea, ammonium salts, nitrogen. 



18. Best media tor long-continued growth 



19. Quick tests for differential purposes 



III. PHYSICAL AND BIOCHEMICAL FEATURES 



. Fermentation-tubes con- 

 taining peptone-water or 

 Sugar-free bouillon and 



Gas_producti on, in per cent 



( coiJ 



Growth in closed arm 



Amount of aci d produced id. 

 Arnount ofaci d produced 2d. 

 Amount of acid produced 4d. 



.ammonia. . . . 

 .free nitrogen. 



Ammonia production, feeble, moderate, strong, 

 absent, masked by acids. 



Nitrates in nitrate broth. 

 Reduced, not reduced. 



Presence of nitrites 



Presence of nitrates . . . 



Indol production, feeble, moderate, strong. 



Toleration of Acids: Great, medium, slight. 



Acids tested 



Toleration of NaOH: great, medium, slight. 

 Optimum reaction for growth in bouillon, stated 

 in terms of Fuller's scale 



8. Vitality on culture media: brief, moderate, long. 



9. Temperature relations: 



Thermal death-point (10 minutes exposure in 

 nutrient broth when this is adapted to growth ot 

 organism) C. 



Optimum temperature for growth . . .C. or 



est growth at I5°C., 2o''C., 2S°C., 30°C., ^^ C.. 

 40<'C., S0°C., 6o°C. 



37°C., 



best growth at IS^C, 

 D'-C, S0°C., eo'-C. 



Maximum temperature for growth o. 



Minimum temperature for growth C. 



10. Killed readily by drying: resistant to drying. 

 It. Per cent killed by freezing (salt and crushed ice or 

 liquid air) 



12. Sunlight: Exposure on ice in thinly sown agar 



plates: one-half plate covered (time is minutes), 

 sensitive, not sensitive. 

 Per cent killed 



13. Acids produced 



14. Alkalies produced 



15. Alcohols 



16. Ferments: pepsin, trypsin, diastase, invcrtase, pectase, 



cytase, tyrosinase, oxidase, peroxidase, lipase, 

 catalase, glucase, galactase, lab, etc 



17. Crystals formed:. .. . 



18. Effect of germicides: 



IV. PATHOGENICITY. 



1. Pathogenic to Animals. 



Insects, crustaceans, fishes, reptiles, birds, mice, rats, 

 guinea pigs, rabbits, dogs, cats, sheep, goats, cattle, 

 horses, monkeys, man 



2. Pathogenic to Plants: 



3. Toxins, soluble, endotoxins. 



4. Non-toxin forming. 



5. Immunity bactericidal. 



6. Immunity non-bactericidal. 



7. Loss of virulence on culture media: prompt, gradual, 



not observed in months. 



