386 



SPECIFIC MICRO-ORGANISMS 



Tube No. 1 



Tube No. 2 



Tube No. 3 



Complement 2 units 



(o.2 c.c.) 

 Patient's serum o.i c.c. 

 Salt solution 0.3 c.c. 



Complement 2 units 



(0.2 c.c.) 

 Patient's serum 0.02 c.c. 

 Antigen i unit (o.i c.c.) 

 Salt solution 0.28 c.c. 



Complement i unit 

 (0.2 c.c.) 

 Patient's serum 0.05 c.c. 

 Antigen i unit (0.1 c.c.) 

 Salt solution 0.25 c.c. 



Mix thoroughly and leave overnight in ice box. Then add: 



Mix thoroughly and incubate at 37° C. in water bath for i hour, recording the 

 progress of hemolysis at intervals of 15 minutes. Then refrigerate 16 hours and 

 record the final reading. 



Tube No. I should show complete hemolysis early in the second 

 incubation. If this has behaved properly and the tests on the 

 known sera have resulted as they did when previously tested, 

 then the behavior of Tubes 2 and 3 is a measure of the amount of 

 lipoid ophilic substance in the serum of the patient. One dis- 

 tinguishes six different grades of reaction, from complete fixation 

 (no trace of hemolysis) to no fixation (complete hemolysis). 

 These are designated by the signs (+ + -f+) (+ + + ) ( + +) 

 (-h) (+) and (-). 



The luetin test is performed by injecting 0.05 c.c. of luetin 

 intracutaneously in two places on the left arm and at the same 

 time 0.05 c.c. of a control suspension, consisting of the medium 



' The suspension of sheep's corpuscles containing i unit in 0.2 c.c. and the solu- 

 tion of hemolytic amboceptor containing 2 units in 0.2 c.c. are quickly mixed to- 

 gether in equal parts, and 0.4 c.c. of this homogeneous mixture is added at this point. 

 This procedure results in a saving of time as well as greater accuracy. 



