CONTENTS. 



PAGE 



Introduction i 



Historical Sketch 8 



PART I. 

 BACTERIOLOGICAL TECHNIQUE. 



CHAPTER I. 



Examination of Bacteria with the Microscope, including Methods of 



Staining iS 



CHAPTER II. 

 Sterilization 4q 



CHAPTER III. 

 Culture-media. , 59 



CHAPTER IV. 

 The Cultivation of Bacteria. Tube-cultures; the Incubator; Anaerobic 



Methods 72 



CHAPTER V. 

 The Cultivation of Bacteria (Continued). Isolation of Bacteria; Plate- 

 cultures 84 



CHAPTER VI. 

 Inoculation of Animals. Autopsies; Collodion Sacs 92 



CHAPTER VII. 

 Collection of Material 97 



CHAPTER VIII. 

 Systematic Study of Species of Bacteria. Suggestions for Class-work; 



Rules loi 



xiii 



