48 MANUAL OF BACTERIOLOGY. 



(/) After a few seconds, replace the preparation in the nitrate 

 of silver solution, in which it is kept constantly moving, till the 

 solution begins to acquire a brown or black color. 



Some recommend leaving the preparation in the nitrate of 

 silver solution for two minutes in the first place, and in the 

 reducing bath for two minutes, without using the nitrate of 

 silver solution a second time. 



(g) Finally wash in distilled water, dry, mount ia Canada 

 balsam. It is difficult to avoid the formation of precipitates; 

 otherwise the results of this method are usually good. 



