INOCULATION OF ANIMALS. 



93 



Guinea-pigs and rabbits, after inoculation, are to be kept in 

 cages of galvanized iron and wire-netting. 

 The bottom may conveniently be made in 

 the form of a movable pan which permits 

 of the disinfection of the excreta. Rabbits 

 and guinea-pigs may be fed with oats, 

 carrots, cabbage, grass and the like. Guinea- 

 pigs and rabbits may be held by an assistant 

 or tied by the legs upon a board. The hair 

 over a small portion of the abdomen is cut 

 away and a short incision is made through the 

 skin; a pocket is produced with a stiff wire, and 

 the material inserted with a sterile platinum 

 wire. The wound may be covered with col- 

 lodion. Sutures may be used if the wound is 

 large. Solid substances may conveniently be 

 introduced by placing them in a sterile glass 

 cannula, which is pushed to the proper situation 

 through a small incision. The substance in 

 the cannula is forced out of it with a stiff, 

 sterile, platinum wire. (Fig. 35.) 



The peritoneal cavity may be inoculated 

 through an incision in the abdominal wall, 

 into which the desired substance may be intro- 

 duced with a sterile platinum wire, the incision 

 being closed with sutures. 



But a more convenient method in many 

 cases, both for subcutaneous as well as intra- 

 peritoneal inoculations, is the use of a hypo- 

 dermic syringe. Material from the surface of 

 solid media can be suspended in sterile beef- 

 broth or physiological salt solution, and cultures 

 in fluid media used directly for these injections. 



Intravenous inoculation is most commonly practiced upon 



Fig. 35- 



