Inoculation of animals. 95 



the surface of the liver; through this surface enter the liver with 

 a sterihzed, platinum wire, and with the material thus obtained 

 inoculate the tubes; also make cover-glass preparations. In 

 the same manner inoculate tubes and make cover-glass prepara- 

 tions from the spleen, the kidneys, the pleural cavity, the peri- 

 cardial cavity, the lungs, and the blood inside the heart and 

 other organs as indicated. If there is a question of the tissues 

 from which the cultures are to be made having become con- 

 taminated, as might be the case where the autopsy is delayed for 

 any reason, it is better to make plates. All incisions are to be 

 made through the burned surfaces, and all material collected 

 for inoculation is to be obtained through burned surfaces. In 

 sterihzing the instruments in the flame avoid sputtering, es- 



D 



Fig. 36. — ^Method op Making Collodion Capsules. — (^Ajter McCrae.) 



pecially when they become covered with oil from adipose tissue. 

 Pieces of lung, Hver, spleen, kidney and other organs, as may be 

 indicated, should be placed in 95 per cent, alcohol or 10 per 

 cent, formalin for fixation and hardening. The animal and 

 the board on which it was extended should be covered with 

 bichloride of mercury solution i-iooo, and afterward burned. 

 The cage or jar and the instruments, dishes and towels used 

 should be steriUzed by steam. The hands of the operator 

 should be washed thoroughly with soap and water and with a 

 i-iooo solution of bichloride of mercury, if there is any pos- 

 sibihty of these having accidentally come in contact with any 

 of the diseased tissues. 



Collodion Capsules. — Bacteria may be cultivated in the 

 Uving body of an animal, without infecting the animal, when 

 they are enclosed in collodion capsules. Their soluble products 



