134 BACTERIOLOGY 



Bouillon. Turbid, with a whitish sediment. 



Milk. Not coagulated. In gelatin and agar much gas. 



Pathogenesis. Mice die in 1-3 days of septicaemia; guinea pigs and rabbits 



refractory. 

 Habitat. Isolated from the blood of a mouse which had been inoculated with 



the sputum of a pneumonia patient. 



29. Bact. limbatum Marpmann 



Bact. Hmbatum-acidi-lacHci Marpmann : Erganzungshefte des Centralblatt f. allgemeine 

 Gesundheitspflege, II, 122. 



Morphology. Bacilli short, thick, with a capsule. 



Milk serum gelatin colonies. 24 hours, punctiform, white, glistening ; edges 



sharp. 

 Gelatin stab. Slight growth in depth ; surface growth white, flat. 

 Litmus milk. 24 hours coagulated, slightly reddened. Grow at 37° C. 

 Habitat. Milk. 



30. Bact. nasalis 



Vorkommen von Frisch'schen Bacillen in der Nasenschleimhaut des Menschen u. der 

 Thiere: Simoni, Centralblatt f. Bakteriol, XXV, 1899,625. 



Morphology. Bacilli rather large, oval, with a thick capsule, which commonly 

 encloses two rods. In cultures smaller, more rod-like, and without a 

 capsule. Stain readily. 



Gelatin colonies. Round, much raised, homogeneous, opalescent, waxy; 

 growth viscous. 



Gelatin stab. Good growth in depth ; no gas. Surface growth raised — convex, 

 opalescent, becoming dirty white, never porcelain-white. 



Agar slant. In 24 hours a moist, glistening, translucent, watery streak. 



Glycerin agar colonies. 24 hours, 37°- Deep : dark, small, opaque. Surface : 

 largest, the size of a pin's head, raised, translucent, whitish-grayish. 



Bouillon. In 24 hours at 37°, a dense turbidity, with a delicate pellicle on the 

 surface. 



Milk. Not coagulated, not acid. 



Potato. A raised, translucent, colorless, watery, glistening streak. No develop- 

 ment in acid media. No gas in glucose bouillon. 



Pathogenesis. Not pathogenic to guinea pigs and rabbits, except an infiltra- 

 tion at the point of injection. 



Habitat. Isolated from nasal secretions in rhinoscleroma. 



