ISO BACTERIOLOGY 



74. Bact. acidiformans Sternberg 



Manual of Bacteriology, 1892, 449. 



Morphology. Bacilli short, thick rods to longer forms and filaments. 



Gelatin colonies. Deep: round, opaque, homogeneous. Surface: round,. 



translucent — opaque, slightly irregular and somewhat iridescent. 

 Gelatin stab. Like Bact. pneu7nonice ; gas in the gelatin. 

 Agar slant. Growth glistening, creamy. In glycerin agar, much gas ;, 



medium becomes intensely acid. 

 Potato. Growth thick, milky white, semi-fluid. 

 Glycerin bouillon. A milky opacity, and much gas. A slight reduction of 



nitrates to nitrites. 

 Litmus milk. Reddened. 

 Pathogenesis. Intraperitoneal inoculations of guinea pigs and rabbits, 1.0-2.0 cc.,. 



cause death in 24 hours ; bacilli in the blood in small numbers ; spleea 



enlarged ; intestines hyperaemic. 

 Habitat. Isolated from the liver of yellow fever cadaver. 



75. Bact. astheniae Dawson 



U. S. Dept. Ag., Bureau of Animal Industry, 1898, 330. 



Morphology. Bacilli 0.5 : i .0-1 .3 ju, ; ends rounded ; occur singly and in pairs.. 

 Do not stain in acid or alkaline methyl-blue or in carbol-fuchsin, but do- 

 stain well in aqueous solutions of the latter. Stain by Gram's method. 



Gelatin colonies. Deep : yellowish, with irregular lobulate margins. Surface r 

 in 24 hours, 1.5 mm., round, raised, papillate in centre, with yellowish, 

 borders. 



Gelatin stab. In depth, yellowish, discrete, closely packed colonies ; on the 

 surface, a brownish, spreading, deeply dentate growth, with delicate 

 periphery. 



Bouillon (acid). A dense turbidity, with pellicle on the surface. Mediuny 

 yellowish green, with a putrefective odor. Alkaline bouillon rendered 

 slightly acid. 



Agar slant. A good, white, opaque growth along line of stroke. 



Potato. In 3 days a yellowish, creamy growth, with gas blisters. 



Glucose bouillon. Rendered acid ; much gas. — — = -. 



Lactose bouillon. Rendered acid ; moderate gas production. ^^^ = -- 



H 2 

 Saccharose bouillon. Rendered acid ; but little gas. p;j=-- =-. 



