THE CLASSIFICATION OF BACTERIA i8j 



CLASS XI. WITHOUT ENDOSPORES. OBLIGATE ANAEROBIC. GELATIN 

 NOT LIQUEFIED. 



I. Grow at room temperatures and in nutrient gelatin. 



A. Gas produced in ordinary nutrient gelatin. 



1 66. Bact. Welchii Migula. 



B. Gas not produced in ordinary nutrient gelatin. 



1 . Large, thick rods, over i .o micron in diameter. 



167. Bact. emphysematosum (Kruse). 



2. Very slender rods, 0.3 micron in diameter. 



168. Bact. infecundum. 



II. No growth at room temperatures or in nutrient gelatin. 



169. Bact. cadaveris Sternberg. 



170. Bact. pyogenes (Kruse). 



166. Bact. Welchii Migula 



Bact. aerogenes-capsulatum Welch-Nuttall : Bull. Johns Hopkins Hospital, July, 1892. 

 Bact. Welchii Migula; System der Bakterien, 1900, II. 



Morphology. Bacilli somewhat thicl<er than anthrax ; chains with a capsule. 

 Gelatin cultures give much gas. 



Gelatin colonies. 1-2 mm., gray white, oval to irregular with a few out- 

 growths. 

 Milk. Coagulated ; has a faint odor of old cheese. 

 Potato. Growth gray white. 

 Pathogenesis negative. 

 Habitat. Isolated from blood in a case of aneurism of the aorta. 



167. Bact. emphysematosum (Kruse) 



B. der Gasphlegmon Fraenkel: Centralblatt f. Bakteriol., XHI, 1893, 13. 

 B, emphysematosus Kruse : Fliigge, Die Mikroorganismen, 1896, 242. 



Morphology. Bacilli somewhat thicker than anthrax ; occur in threads. 

 Stained by Gram's method. 



Gelatin. With addition of glycerine and formate of soda, active growth with 

 much bad-smelling gas. 



Glucose bouillon. Much gas. 



Bouillon. Turbid. 



Pathogenesis. Guinea pigs, by subcutaneous inoculation, a local non-sup- 

 purating inflammation; later, a necrosis sometimes spreading to perito- 

 neum and pleura. 



Habitat. Isolated from gaseous phlegmon. 



