THE CLASSIFICATION OF BACTERIA 20^ 



Agar slant. Growth thin, glistening, spreading ; condensation water turbid. 



Potato. Growth brownish yellow, becoming thicker and brown ; not viscid. 



Alkaline bouillon. Densely turbid ; grayish sediment ; acid, becoming alka- 

 line. In old cultures, 3-6 weeks ; a grayish pellicle on the surface. 



Milk. Coagulated in about 3 days at 37° ; serum clear ; casein not digested ; 

 acid ; odor sour. 



Glucose bouillon. Gas, maximum in 2 days ; growth in both arms ; acid. 



Lactose bouillon. Gas. 



Saccharose bouillon. No gas ; closed arm clear, remains alkaline. H : COj : : 

 2:1. Indol produced. 



Habitat. Isolated from tainted, gassy, ckeesy curd. 



4. B. anindolicum Lembke 



Archiv f. Hygiene, XXVII, 1896, 384. 



Morphological and cultural characters like B. coli ; differs in producing an 

 amount of acid in milk intermediate between B. coli and B. typhosus. 



Pathogenesis. Inoculations of 0.2 cc. of bouillon culture subcutaneously into 

 mice cause death in 24 hours, with general septicaemia. Guinea pigs die 

 by intraperitoneal injections of 0.7 cc. of bouillon culture. 



Habitat. Isolated from the faeces of a dog. 



5. B. enteritidis Gartner 



Correspond, d. allg. Artzl. Vereins, Thuringen, 1888. 



Morphology. Bacilli short, thick ; stain unequally ; capsule present or absent. 



Gelatin colonies. Deep : brown. Surface : round, gray, translucent, granular. 



Lactose bouillon. Gas. 



Potato. Growth grayish white to grayish yellow, glistening. 



Pathogenesis. Pathogenic to mice, guinea pigs, rabbits, pigeons, young sheep, 



and goats. Non-pathogenic to dogs, cats, rats, chickens, and sparrows. 



Mice and guinea pigs infected through the stomach, producing enteritis ; 



bacilli found in the organs. 

 Habitat. Isolated from beef in meat poisoning. 



6. B. chologenes Kruse 



Discovered by Stem, no name : Deutsche med, Wochenschrift, 1893, No. 26. 

 B. chologenes Kruse : Flugge, Die Mikroorganismen, 1896, 374. 



Morphology. Bacilli 0.5-1.3 ;u... 



Gelatin colotties. White ; border erose ; characters between the colon and 

 aerogenes types. 



I I 



