THE CLASSIFICATION OF BACTERIA 211 



Milk. Not coagulated, reaction unchanged or alkaline; medium rendered, 

 opalescent. 



Glucose bouillon. Gas , reaction acid. According to Karlinski, gas is pro- 

 duced in glucose bouillon with bacilli fresh from the body, but is incon- 

 stant in cultures. No gas in saccharose bouillon. 



Litmus milk. Unchanged, or a deeper blue. Indol and phenol not produced. 



Pathogenesis. Pathogenic to mice and rabbits; death in 7-12 days; spleen 

 enlarged, in liver necrotic spots, kidneys inflamed, bacilli in the organs. 

 More attenuated varieties cause only an infiltration and ulceration of 

 Peyer's patches and an infiltration of lymph glands. 



Habitat. Associated vi^ith hog cholera. For variations of this species, see 

 Smith: U. S. Dept. of Ag., Bureau of Animal Industry, Bull. 6, 1894, 

 pp. 8-27. 



14. B. levans Lehmann-Wolfifin 



Archiv f. Hygiene, XXI, 1894. 



Morphology. Bacilli 0.6 : i .8 /n, with numerous long fiagella. Cultural char- 

 acters like B. coli. 



Glucose bouillon. Gas ; H : COj: : 1 : 3. No gas in saccharose bouillon. Lactic 

 acetic and butyric acids in glucose bouillon. 



Habitat. Isolated from sour dough. 



15. B. loxiacida Tartakowsky 



Archiv d. Veterinarwissenschaft, 1888. 



Morphology. Bacilli 0.6-1.0: 2.0-2.5 /u,. Not .stained by Gram's method. 



Gelatin colonies. Surface : 2 mm., round to irregular, with entire borders ; 

 microscopically, gray-brown, radiately fibrous to granular on the border. 

 Colonies become crumpled when dry. 



Gelatin stab. A slight amount of gas in depth. 



Agar slant. Growth abundant, moist, white. 



Bouillon. Turbid, with an easily disturbed membrane. 



Potato. A scanty growth. No growth on acid potato. 



Blood serum. Growth moist, white. 



Milk. Not coagulated. 



Pathogenesis. Pathogenic to birds. Subcutaneous inoculation of guinea pigs 

 cause a slight local swelling, with elevation of temperature. Intraperi- 

 toneal injections cause sero-fibrinous peritonitis and death in 1-2 days. 



Habitat. Associated with an infectious disease of titmouse, crossbill, gold- 

 finch, and canary birds. 



