THE CLASSIFICATION OF BACTERIA 225. 



Gelatin stab. Growth filiform in depth ; surface growth white, scarcely- 

 visible, covers the entire surface. 



Gelatin slant. A limited yellowish white stripe, with a thin colorless border,, 

 and with outgrowths. 



Agar colonies. In 1-2 days at 30°, the deep colonies are small, white, and 

 entire; the surface colonies thin, soft, colorless, limited, with erose 

 borders. 



Nitrate bouillon. A membrane on the surface ; reduction to nitrites. 



Habitat. Straw, earth, and horse manure. 



59. B. Stutzeri (Lehmann-Neumann) 



B. denitriftcans, II, Burri-Stutzer : Centralblatt f. Bakteriol., IV, 1898. 

 Bact. Stutzeri Lehmann-Neumann, Bak. Diag., 1896, 2te Abl., 1898, 408. 



Morphology. Bacilli ; on agar rods with rounded ends, often spindle-shaped,- 

 0.5-1.0 : 1.5-2.5-4 ju, ; in bouillon, only 0.25-0.3 ft. thick. Polar stain. 



Gelatin colonies. In 4 days the deep colonies are small, white, entire ; the 

 surface colonies thin, whitish, translucent, with somewhat ragged edges. 



Agar colonies. In 2 days at 30° the deep colonies are small, yellowish white, 

 entire; the surface colonies thin, 5-10 mm. 



Agar slant. Growth soft, flat, dry, grayish, becoming rather moist, glistening. 



Gelatin slant. Growth thin, milky-white, with fine radial striping and 

 undulations. 



Gelatin stab. In depth growth pearly; on the surface growth milky-white, 

 soft, dry, becoming somewhat slimy. 



Nitrate bouillon. A weak turbidity ; in 2 days a membrane. Nitrates- 

 reduced. 



Habitat. Straw, earth, air. 



60. B. centropunctatus (Jensen) 



Bad, centropunctatum Jensen : Centralblatt f. Bakteriol,, IV, 

 2te Abt., 1898, 410. 



Morphology. Bacilli ; on agar coccoid, 0.3-0.5 ;«., with a capsule. In nitrate- 

 bouillon, morphology like B. Stutzeri. In anaerobic cultures bacilli large, 

 ovoid, 0.5-1.0 fx.. 



Gelatin colonies. In 4 days the deep colonies are small, white, entire; the 

 surface colonies thick, moist, glistening, grayish, becoming whitish. 



Agar slant. Growth thin, soft, becoming in 2 days thick, glistening, grayish,, 

 slightly raised in the middle. 



Gelatin slant. Growth milky-white, slimy, glistening, with long outgrowths. 

 Q 



