270 ' BACTERIOLOGY 



Agar slant. Growth thin, greenisb white, translucent. 



Gelatin stab. Slight growth in depth, with later spherical outgrowths ; sur- 

 face growth capitate, siinken in the gelatin ; evaporation exceeds lique- 

 faction. 



Potato. In 3 days, a bluish discoloration along the line of inoculation, 

 becoming yellowish, moist, glistening. 



Bouillon. A slight turbidity, becoming clear. 



Pepton rosolic acid solution. Decolorized. 



Litmus milk. Alkaline, not coagulated. In two weeks becomes translucent- 

 and violet. 



Glucose bouillon. No gas. Indol negative. Optimum temperature 36°. 



Habitat. Soil. 



170. B. alpha Dyar 



I.e., 366. 



Morphology. Bacilli large, 0.8 : 1-2 /x ; occur singly and in chains. 



Gelatin. Not liquefied for 1 1 days or more. 



Agar slant. Growth translucent, glistening, white. 



Litmus milk. Not coagulated ; not reddened. Reduction of nitrates negative 



or slight. /«flfa/ production slight. 

 Habitat. Air. 



171. B. beta Dyar 



I.e., 366. 



Morphology. Bacilli short, rounded, 0.6: 1.5-2.0 /a. 



Gelatin. Liquefied slowly. Surface : growth thin, feathery. 



Agar slant. Growth translucent, glistening, white. 



Litmus milk. Not coagulated ; not reddened. Reduction of nitrates negative 



or slight. Indol production slight. 

 Habitat. Air. 



172. B. Pammelii 



B. gasoformans Pammel : Centralblatt f. Bakteriol., ate Abt., II, 1896, 642. 



Morphology. Bacilli 1.2-1.4:3.7-4 /t, variable; in chains of 2-5. Motihty 



slight. 

 Gelatin colonies. Lenticular. 

 Gelatin slant. Growth white, limited. 

 Gelatin stab. Liquefaction infundibuliform. 

 Agar slant. Growth white, wrinkled. 

 Bouillon. Good growth, with a pellicle. 

 Potato. Slightly granular, large wrinkles, spreading. 



