THE CLASSIFICATION OF BACTERIA 277 



Gelatin colonies. Crateriform, turbid ; microscopically, round, entire, becom- 

 ing ciliate ; dense flocculi in the liquefied gelatin — felted, floccose, 

 densest in the centre. 



■Gelatin stab. Liquefaction crateriform — saccate — stratiform, with a pellicle 

 on the surface. 



Agar colonies. Anthrax-like. 



Bouillon. Turbid ; a membrane on the surface adhering to the walls of the 

 tube. 



Totato. Growth white, thick, mealy. 



Agar slant. Growth thick, crumpled. 



Milk, Coagulated, peptonized, slightly alkaline. Indol negative. 



Habitat. Widely distributed. 



195. B. lactis-albus Sternberg 



Described but not named Loffler ; Berliner klin. Wochenschrift, 1887, Nos. 33-34. 

 B. lactis-albui Sternberg: Manual, 1892, 680. 



As far as described like the preceding. In milk, the production of butyric 



acid, leucin, and tyrosin. 

 Habitat. Milk. 



196. B. leptosporus Klein 



Centralblatt f. Bakteriol.. VI, 1889,6. 



In fluid media like B. subtilis. Characters on solid media not described. 



Spore germination polar. (See Fig. 6.) 

 Habitat. A culture contamination. 



197. B. megatherium De Bary 



Vergl. Morph. Phys. u. Biol, der Pilze, Strassburg, 1884. 



Morphology. Bacilli very large, approaching 2-5 microns in thickness ; in 

 cultures, after successive generations much smaller, 0.6-0.8 ju,, somewhat 

 bent and curved. Numerous peritrichic flagella. Aerobic. Stain- by 

 Gram's method. 



Gelatin colonies. Deep colonies gray white, translucent, denser in the centre, 

 granular, ciliate. Surface colonies raised, centres dense, borders ciliate. 



Gelatin stab. Liquefaction saccate ; no membrane. 



Agar colonies. Elevated, white, grayish, moist, glistening; microscopically, 

 segmented. 



Agar slant and potato. Growth like B. subtilis, slightly yellowish. Indol 

 negative. H2S positive 



Habitat. Isolated from cabbage infusion. 



