THE CLASSIFICATION OF BACTERIA 28 1 



Bouillon. Turbid, with a membrane, becoming clear. 



Agar slant. Growth gray, smooth, not floccose or crumpled. 



Potato. Growth gray-white, soft, becoming moist, glistening. 



Milk. Coagulated, amphoteric. 



Pathogenesis. Non-pathogenic to mice. 



Habitat. Isolated from South American bran. 



208. B. vaculatus Ravenel 



I.e., 31. 



Morphology. Bacilli straight, with rounded ends ; occur singly ; stain like 



Klebs-Loffler bacillus. 

 Gelatin colonies. Deep colonies : yellowish, granular, entire. Surf ace colonies : 



in 2 days, 0.5 mm., yellowish, entire, granular, mottled; liquefaction 



crateriform. 

 Gelatin stab. Surface growth raised ; in 3 days, a crateriform liquefaction, 



becoming stratiform, with a membrane. 

 Agar slant. Growth thin, translucent, moist, glistening ; agar stained a faint 



greenish. 

 Potato. Growth light brown, slimy, thick, moist, becoming yellowish brown. 

 Bouillon. Turbid, becoming clear. 

 Pepton rosolic acid solution. In 12-14 days, a cherry-red. 

 Litmus milk. Rendered alkaline, becoming watery, translucent, decolorized, 



peptonized, alkaline ; not coagulated. 

 Glucose bouillon. No gas. Indol positive. Grow at 36°. 

 Habitat. Soil. 



209. B. Fliiggei 



B. lactis No. i Flugge: Zeitsch. f. Hygiene, XVII, 1894. 



Morphology. Bacilli thick, short. 



Gelatin colonies. With outgrowths. 



Agar slant. Growth grayish white. 



Potato. Growth gray-white. 



Bouillon. Turbid, with a flocculent sediment. 



Milk. Peptonized, toxic. 



Habitat. Milk. 



210. B. crinatus 



B. No. V Pansini : Virchow's Archiv, CXXII. 



Morphology. Bacilli rather more slender than B. subtilis. 

 Gelatin colonies. Coli-like, with fine, undulating radiations. 

 Gelatin stab. Liquefaction saccate. 



