THE CLASSIFICATION OF BACTERIA 283 



Glycerin gelatin stab. Growth in depth, slender, jagged ; on the surface, a 

 bluish membrane in the form of a funnel. 



Agar slant. At 36° growth bluish white. 



Potato. Growth at first typhoid-like, becoming a granular membrane. 



Pathogenesis. Subcutaneous inoculations of guinea pigs cause death in 1-2 

 weeks. There is emaciation, paralysis of the sphincter muscles, nodular 

 elevations on the mucous wall of stomach, etc. 



Habitat. Isolated from growths on the stomach of a person who died of can- 

 cer of the stomach. 



213. B. AfanassieflB Trevisan 



B. des Keuchhustens Afanassieff: St. Petersburg med. Wochensch., 1887, No. 39-42. 

 B. Afanassieffl Trevisan : Genera, 1889, 13. 



Morphology. Bacilli 0.6-2.2 /x; solitary in pairs and in short chains. 

 Gelatin colonies. Round — oval, light brown ; microscopically, finely granular 



and dark brown. 

 Gelatin stab. Slight growth in depth ; on the surface the growth is grayish 



white. 

 Agar slant. Growth thick, gray, limited. 

 Potato. Growth yellowish glistening dew-like drops, becoming thicker, 



brownish, and spreading. 

 Pathogenesis. Inoculations into the air passages and pulmonary parenchyma 



of young dogs and rabbits cause bronchial catarrh, bronchopneumonia, 



and sporadic coughing ; bacilli in the bronchial and nasal secretions. 



214. B. Cladoi Trevisan 



Bacille peduncule Clado : Bull. Soc. Anat., Paris, 1887, 339, 



B. Cladoi Trevisan : Genera, 1889, 14. 



B. septicus-vesictB Sternberg: Manual, 1892, 475. 



Morphology. Bacilli 0.5 : 1.6-2.0 ju.; never in pairs — chains. Stain by 

 Gram's method. Facultative anaerobic. Grow at 36°. 



Gelatin colonies. Do not exceed 1.2 mm., round, oval, transparent, yellowish 

 white — dark gray, with a zone of yellow. 



Gelatin stab. Surface growth thin, jagged. 



Agar slant. Growth scanty, a grayish yellow stripe. 



Potato. Growth flat, dry, brown. 



Pathogenesis. Inoculations of mice, guinea pigs, and rabbits cause toxaemia 

 and septicaemia. 



Habitat. Isolated from urine of a person with cystitis. 



