292 BACTERIOLOGY 



CLASS XX. WITH ENDOSPORES. OBLIGATE ANAEROBIC. RODS NOT 

 SWOLLEN AT SPORULATION. 



Malignant Oedema Group. 

 I. Gelatin liquefied. 



A. Gas developed in gelatin or agar media containing sugar. 



1 . Colonies on gelatin compact, dense. 



a. Strongly pathogenic. 



239. B. mdeniatis Zopf. 



b. Negatively, or but slightly pathogenic. 



240. B. pseudcedeniatis Kruse. 



2. Colonies on gelatin radiately filamentous or mycelioid. 



241. B. radiatus Liideritz. 



242. B. thalasophilus Russell. 



B. No gas development in gelatin or agar media containing sugar. 



1 . Gelatin liquefied. 



243. B. caris. 



2. Gelatin not liquefied. 



a. Gelatin colonies roundish, dense, entire, not arborescent or 

 mossy. 

 * Gas produced in nutrient gelatin without sugar (glucose). 



244. B. amylozyma Perdrix. 



** No gas produced in nutrient gelatin without glucose. 



245. B. solidtis Luderitz. 



246. B. tardus. 



6. Gelatin colonies mossy, with moss-like offshoots. 



247. B. muscoides Liborius. 



239. B. oedematis Zopf 



Vibrio seftique Pasteur: Compt. rend., LXXXV, 1877. 



(Edema Bacillus Koch : Mitteilungen Kaiserlich. Gesundheitsamte, 1881. 



B. cedematis-maligni Zopf: Spaltpilze, 1885, 88. 



Morphology. Bacilli 0.8-1 .0 : 2-10 /x ; ends rounded, also forms approaching 

 anthrax ; occur singly, in chains and in filaments. Not stained by 

 Gram's method. 



Gelatin colonies. Like B. subtilis. 



Agar colonies. Composed of a dense network of threads. 



Gelatin stab. Below the surface a white line, with short outgrowths, and gas. 



Bouillon. Turbid, gas. 



Litmus milk. Coagulated slowly or not at all, amphoteric, decolorized in 

 depth. Indol slight. 



