THE CLASSIFICATION OF BACTERIA 337 



Bouillon. Turbid, with a thin pellicle. 



Pathogenesis. Non-pathogenic to guinea pigs. 



Habitat. Well water. 



{B) Vibrio choleroides, ^. Bujwid: Centralblatt f. Bakteriol., XIII, 1893, 120. 



Morphological and cultural characters like Msp. comma, but more anaerobic 



in habit, and forms a deeper liquefied funnel. 

 Habitat. Water. 

 (C) Vibrio Ivanoff Kruse : Flugge, Die Mikroorganismen, 1896, 592. Noted 



by Ivanoff: Zeitsch. f. Hygiene, XV. 

 Bacilli fine and elongated, slightly curved comma forms ; otherwise identical 



with Msp. comma. Ivanoff considers it only a morphological variety. 

 Habitat. Isolated from the dejecta of typhoid patients. 



7. Microspira danubica (Haider) 



Vibrio danubicus Heider: Centralblatt f. Bakteriol., XIV, 1S93, 341. 



Morphology. Bacilli like Msp. comma. Gelatin rapidly liquefied, crateriform 



— funnel-formed. 

 Gelatin colonies. When the gelatin layer is thick, colonies like Msp. comma; 



when thin, surface colonies flat, spreading, irregular, with undulate — 



coarsely lobate borders. 

 Milk. Coagulated. 

 Potato. At 37° growth brownish, scanty. Indol positive. Pathogenic for 



guinea pigs, slightly so for pigeons. A negative reaction with Pfeiffer's 



serum. 

 Habitat. Isolated from canal water. 



8. Microspira Berolinensis (Neisser) Migula 



Vibrio Berolinensis Neisser : Archiv f. Hygiene, XIX, 1893. 



Morphology. Bacilli like Msp. comma, but somewhat smaller. A single 

 polar flagellum. Not stained by Gram's method. 



Gelatin colonies. Like Msp. comma, but not much granulated or fragmented. 

 Liquefaction of the gelatin very slight. Deep colonies : small, round, en- 

 tire, colorless, transparent, slightly granular. Surface colonies: small, 

 thin, membranous, with a round central nucleus ; no depression is formed, 

 and the edges remain sharp. 



Gelatin stab. Growth like Msp. com-ma, but a slower growth. 



Agar slant and potato. Growth like Msp. comma. 

 z 



