THE CLASSIFICATION OF BACTERIA 339 



"Varieties. — 



(A) Vibrio Lissabonensis Pestana-Bettencourt : Centralblatt f. Bakteriol., XVI, 

 1894, 401. 



According to Chantemesse identical, or nearly so, with the preceding. From 



descriptions, indistinguishable from the latter. 

 .Habitat. Isolated from a case of choleraic disease in Lisbon. 



(B) Vibrio helicogenes Fischer: Centralblatt f. Bakteriol., XIV, 1894, 73. 

 From descriptions, indistinguishable from Msp. protea. 



Habitat. Isolated from the stools of a sick woman. 



II. Microspira Gindha (Kruse) 



Vibrio Gindha Kruse : Flugge, Die Mikroorganismen, 1896, 590. 



Morphology. Bacilli rather long curved rods, rather smaller than Msp. 

 comma. A single polar flagellum. 



Gelatin colonies. Cholera-like. In pepton solution in 24 hours, at 37°, a 

 negative or only a faint indol reaction. 



J'athogenesis. Subcutaneous inoculations into pigeons and guinea pigs nega- 

 tive. Living or dead cultures show toxic properties when inoculated 

 intraperitoneally into guinea pigs. 



Habitat. Isolated by Pasquale from water. 



12. Microspira aquatilis (Gunther) 



Vibrio aquatilis Gunther: Deutsche med. Wochenschrift, 1892, 1124. 



Morphology. Bacilli like Msp. comma. A single polar flagellum. 



Gelatin colonies. Round, entire, brownish, finely granular. 



Gelatin stab. Growth only on the surface, becoming a crateriform liquefaction. 



Agar slant. Growth like cholera. 



Potato. No growth either at 20° or 37° 



Bouillon. No growth in alkaline or amphoteric bouillon at 37° ; at 20°, only a 



faint growth after some weeks. Tndol negative. A strong odor of HjS. 



Non-pathogenic. 

 Habitat. Isolated from water of river Spree. 



13. Microspira tyrogena (Dencke) Migula 



spirillum tyrogenum Dencke : Deutsche med. Wochenschrift, 1885, No. 3. 

 Msp, tyrogena Migula : I.e. 



Morphology. Bacilli like Msp. comma, but rather smaller and more slender, 

 often very long spirals with close windings. 



