344 BACTERIOLOGY 



Pathogenesis. Subcutaneous and intramuscular inoculations of pigeons and 



guinea pigs fatal. 

 Habitat. Isolated from dejecta of cholera patients. 



3. Spirillum serpens (Miiller) Winter 



Vibrio serpens Miiller : Animalia infusoria, 1786. 



Spirillum serpens 'Wmiti : Die Pilze, 18S4. Zettnow: Centralblatt f. Bakteriol., 

 X, 689. 



Morphology. Bacilli rigid filaments, with 2-3 wave-like undulations, 0.8-1.0 : 

 10-30 jx.. Polar flagella in bundles of 14. Spores absent. 



Gelatin colonies. Typhi- or coli-like. 



Gelatin stab. Growth coli-like ; surface growth becomes sunken, with the 

 formation of a bubble. 



Agar slant and potato. Growth like B. coli. 



Bouillon. Turbid, with a delicate membrane. 



Habitat. Isolated from stagnant water. 



4. Spirillum tenerrimum Lehmann-Neumann 



spirillum I Kutscher: Zeitsch. f. Hygiene, XX, 1895, 47. 

 Spirillum tenerrimum Lehmann-Neumann : Bale. Diag., 1896, 346. 



Morphology. Bacilli short forms ; as a rule, thin. Spirilla of 3-4 elements. 



Gelatin colonies. Show a compact centre, a thin, finely granular zone, and an 

 outer filamentous border. 



Gelatin stab. Growth like that of the bacillus of mouse septicaemia, liquefac- 

 tion slow. 



Agar slant. Growth of dewdrop colonies. 



Bouillon. Slightly turbid — no membrane. The above may be a Microspira. 



Habitat. Sewage. 



5. Spirillum undula (Miiller) Ehrenberg 



Vibrio undula Miiller: Historia Vermium terrestrium et fluviat Haunise, 1773. 

 Spirillum undula Ehrenberg: Abhandl. Ber. Akad., 1830, 38. 



Morphology. Bacilli stout threads, i. 2-1. 5 : 8-16 jit, one-half to three turns. 



Wave length, 4-; /x,. Bundles of 3-9 flagella at both poles. Spores 



present (Migula, I.e.). 

 Gelatin colonies. Deep : small, entire, granular ; no growth on the surface. 

 Gelatin stab. A development in the upper part of the stab ; on the surface, 



growth thin, white, somewhat rugose. 

 Potato. No growth. 

 Bouillon. Turbid ; no membrane. 

 Habitat. Putrid and stagnant water. 



