go THE DEATH-POINT OF 
viously been well rinsed with. boiling water. A 
number of glass bottles or tubes were also prepared, 
which, together with their stoppers or corks, had 
been boiled in ordinary tap water for a few minutes.* 
They were taken. out full of the boiling fluid; and 
the stoppers or corks being at once inserted, the 
vessels and their contents were set aside to cool. 
When the filtered infusion of hay or turnip had 
been rapidly cooled down. to. about 110° F. (by 
letting the beaker containing it stand in a large 
basin of cold water), it was inoculated with some 
of a turbid infusion of hay swarming with active 
Bacteria and Vibriones—in the proportion of one 
drop of the turbid fluid to each fluid ounce of the 
now clear filtered infusion.t The beaker was then 
placed upon a sand-bath, and its contained fluid 
(in which a thermometor was immersed) gradually 
raised to the required temperature. The fluid 
was maintained at the same temperature for five 
minutes by alternately raising the beaker from and 
replacing it upon the sand-bath. The bottles to 
be used were then one by one uncorked, emptied, 
* The vessels employed have varied in capacity from two drachms to 
four ounces ; some have been provided with glass stoppers and others 
with very tightly fitting corks; and the latter I find have answered quite 
as well as the former. On the whole I have found tightly corked one- 
ounce phials to be about the most convenient vessels to employ in these 
inoculation experiments. 
+ It was found desirable to filter the infusions after they had been 
boiled, because the boiling generally somewhat.impaired their clearness. 
