444 PRACTICAL DIRECTIONS. [APP. 



G. The following transverse sections will perhaps be 

 the most instructive. 



Manipulation as ia I. B. 3. 



1. Througli the optic vesicles, shewing the optic 

 stalks. 



2. Through the hind-brain, shewing the auditory 

 sacs. 



3. Through the middle of the heart, shewing its re- 



lations to the splanchnopleure and alimentary canal. 



4. Through the point of divergence of the splanch- 

 nopleure folds, shewing the venous roots of the 

 heart. 



5. Through the dorsal region, shewing the medullary 

 canal, niesoblastic somites and commencing cleavage 

 of the niesoblast. 



6. Through a point where the medullary canal is still 

 open, shewing the mode in which its closing takes 

 place. 



Longitudinal sections should also be made and 

 compared with the transverse sections. 



III. Examination of an Embryo of about 48—50 hours. 



A. Opening the egg — as in II. A. 



B. Examination of the blastoderm in situ. 

 Observe 



1. The ybrm of the embryo, which is much more dis- 

 tinct than at the earKer stage. 



2. The healing of the heart. 



3. The genoral features of the circulation. 



