124 STORRS AGRICULTURAL EXPERIMENT STATION. 
curdles and digests it. The acidity in sugar bouillon is less pronounced than 
in the other varieties, and in lactose there is usually no acidity. The litmus 
gelatine colonies are usually acid, though not always. In other respects they 
agree with the type. 
M. Freudinreichii of Guillebeau appears to belong here, differing from those 
mentioned above in rendering milk sdmy. 
THE GENUS SARCINA. 
We have not found the genus sarcina very commonly represented in milk 
products. The cultures which we have found may all be reduced to four types; 
for, while the different specimens show some variations, they are not very great, 
and not so great but what they may be properly included under one of the four 
types. 
Sar, lactis albusn.s. A white or yellow non-liquefying Sarcina. 
Morphology.—Size; .74. The Gram stain is positive and there is no motility. 
Gelatine colony.— Round, convex, smooth, homogeneous, entire, yellowish or 
white. — 
Gelatine stab.—A needle growth and a convex surface growth, 
Agar streak.— Beaded, raised, smooth, translucent, cream-white, moist, not 
luxuriant, 
Fermentation tubes.—Acidity and closed arm growth in all sugar bouillons, 
but no gas. Sometimes lactose shows no closed arm growth. 
Boutllon,—A sediment and a slight turbidity, but no pellicle. 
Milk.—Becomes sufficiently acid to curdle on boiling. No other change. 
Potato.—A very slight cream-white growth. 
Grows at 20° but scarcely at all at 37° Facultative anaerobic. 
. Sar, lactis lutean.s. A yellow, liguefying Sarcina, Resembles Sar. lutea 
of Fligge. 
Morphology.—Size, .74-1p. Gram stain positive. Not motile. 
Gelatine colony.—A slow liquefying pit, with a nucleus surrounded by 
granular area; yellowish. Litmus gelatine not acid. 
Gelatine stab.—Begins to liquefy in about three weeks, crateriform, and never 
complete. 
Agar streak.—Filiform, raised, smooth, opaque, lemon-yellow, luxuriant. 
fermentation tubes.—No acidity, no gas, no closed arm growth in any sugar 
bouillon. 
Boutllon.—A sediment, but no turbidity nor pellicle. 
Milk.—Becomes alkaline and slowly digests, with a yellow color, but does 
not curdle. 
Potato.—Beaded, raised, opaque, lemon-yellow, luxuriant. 
Grows at both 20° and 37° _— Aerobic. 
Among the variations found in our several cultures the following may be 
mentioned: Size, from .74-24; color, lemon-yellow to whitish; the reaction on 
milk is frequently not changed; the growth on potato ranges from luxuriant to 
no growth. 
