CLASSIFICATION OF DAIRY BACTERIA. 145 
Bact, lactis cretaceum n. >. A non-arborescent, spore-bearing Bacterium. 
Morphology.—Size, 3-5" x 1.4u. Nochains nor capsules. The Gram stain 
is positive. Spores are produced, no larger than the rods. 
Gelatine colony.—Not characteristic; a rather slowly liquefying pit forms, 
without any distinguishing marks. In /¢mus gelatine it is not acid. 
Gelatine stab.—No needle growth. In one day it begins to liquefy; strati- 
form or sometimes infundibuliform. 
Agar streak.—Filiform, raised, smooth, cretaceous, white or flesh-colored, 
moist, luxuriant. 
Fermentation tubes.—Acidity and closed arm growth in dextrose and saccha- 
rose; closed arm growth, but not acid inlactose. No gas. 
Bouillon.—A sediment, a slight turbidity and, later, a slight pellicle. 
Milk.-—Becomes alkaline, curdles promptly and completely digests, with a 
_ Prominent odor. 
Potato.—Spreading, raised, smooth, cretaceous, white, luxuriant; potato dis- 
colored. 
Grows at 20° and 37°. Facultative anaerobic. 
Bact, lactis lobatum n.s. An orange, acid liquefier. This organism was 
found in stable dust and in milk direct from the udder. The two cultures 
differ slightly in color and bouillon growth. 
Morphology.—Size, .84-1m x .54. No chains, no spores, no capsules. Gram 
stain positive. 
Gelatine colony.—A round, raised, smooth homogeneous coijony, sometimes 
yellowish, with a lobate center and a clear ring when liquefying. Litmus gelatine 
shows an acid, liquefying colony. 
Gelatine stab,—Slow liquefier, at first saccate, and then stratiform. The 
liquid is cloudy. 
Agar streak.—A smooth, raised, thin, orange-colored colony, luxurious. 
Fermentation tubes.—All three sugars are rendered acid, and there is growth 
in the closed arm, but no gas. 
Bouillon.—A sediment, turbidity, and pellicle. In one culture there is no 
pellicle. 
Milk.—Rendered acid, but not curdled. It is digested and turned amber- 
color or reddish, with a jelly-like sediment. 
Potato.—Thick, opaque, orange-colored, luxurious. 
Grows well at 20°, hardly at all at 37° Facultative anaerobic. 
Bact. lactis cloacae n.s. This organism appears much like B. cloacae of 
Jordan, differing slightly in its growth in bouillon and on potato. Its lique- 
fying power is also less. It was found only once in milk, in Middletown. 
Morphology,—Size, .7#-.8h X 5M. No chains, no spores. Gram stain nega- 
tive. It shows a capsule, 
