CLASSIFICATION OF DAIRY BACTERIA. 149 
Variety A.—Isolated from milk in Connecticut. Is not quite so large. Gram 
stain negative. Saccharose is not acid. Produces iridescent brown color. 
Does not grow well at 20°. Milk has a peculiar color. 
Variety B.—(From milk in New York city.) Saccharose is acid. There is 
no pellicle on bouillon. No digestion of milk. Growth on potato is scanty. 
Variety C.—(From Brie cheese.) Has a simple, granular, rapidly-liquefying 
colony. Shows growth in closed arm of fermentation tubes, and a pellicle on 
bouillon, The milk develops the odor of Brie cheese. 
Varieties A and B are probably cultures of the same organism, one of 
which is more vigorous than the other. Here evidently belongs Bact. C. of 
Miller (Arch. f. Hyg. LXVII., p. 127), which agrees in all points, but shows a 
blue fluorescence in gelatine, and gas in dextrose bouillon. 
Bact. lactis magnum n.s. A non-acid, white, hguefying Bacterium, 
Morphology.—Size, 34x 1.5. Chains produced; no spores or capsules. 
Gram stain positive, Rods with square ends. 
Gelatine colony.—A fairly rapidly liquefying pit, which may be filamentous 
and with ciliated edge. On ditmus gelatine it is not acid. 
Gelatine Stab.—A needle growth, which may be arborescent and later lique- 
fying; stratiform. Liquefaction begins in one to three days; complete in three 
weeks. 
Agar streak.—Filiform or spreading, thick, punctate, opaque, white, moist, 
luxuriant. 
Fermentation tubes.—Acid in dextrose only. No closed arm growth and no 
gas. 
Bouillon.—A sediment, a turbidity, and a pellicle. 
Milk.—Becomes alkaline, curdles after three days and digests into a brownish 
liquid, and a prominent odor. 
Potato.—Spreading, thick, contoured, translucent, white; potato discolored. 
Grows at 20° and 37°. Aerobic. 
Bact. lactis flocculus u.s. An acid, non-curdling, liquefying Bacterium., 
From Camembert cheese. 
Morphology.—Size, 1u-2¢ x TM. No chains, no spores, Gram stain positive. 
Gelatine colony.—A slowly liquefying colony, which is lobate or moruloid. 
Gelatine stab,—A needle growth and a surface growth, which begins to liquefy 
in ten days. 
Agar streak.—A filiform, raised, smooth, opaque, white colony, rather 
scanty. 
Fermentation tubes.—Dextrose ts acid, but no other sugar bouillon. No 
closed arm growth, no gas. One culture is acid in all sugars. 
Bouillon.—A sediment, turbidity, and a pellicle. One culture shows no 
growth. : 
Ailk.—Acid but not curdled, and shows no digestion. Hasa prominent odor. 
