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CLASSIFICATION OF DAIRY BACTERIA. 159 
Milk,—Rendered acid, curdled, and digested at 20°, without change in color. 
Potato.—Grows well. 
Grows at 20° and 37°. 
Ps, lactis granulan. s. A non-curdling, liquefying monotrich. Found in 
New York city milk and also sent by Harding. 
Morphology.—Size, .74x 24. A rod, forming chains. There are no capsules, 
and the Gram stain is negative. Spores are produced. 
Gelatine colmy.—A rapidly liquefying pit is formed, which is uniformly 
coarsely granular, and has a ciliated margin. 
Gelatine stab.—A spiny needle growth and a napiform pit, which later is 
stratiform. Liquefaction begins in one day. 
Agar Streak.—A filiform, pie smooth, grayish, moderately luxuriant 
growth. 
Fermentation tubes.—Acidity is produced in all three bouillons, but no gas 
nor closed arm growth. 
Bouillon.—A sediment, an abundant turbidity, and a membranous pellicle. 
Milk.—Rendered slightly alkaline, but no other change seen. 
Potato.—Shows no growth. 
Grows hardly at all at 20°, but abundantly at 37°. Aerobic. 
THE GENUS BACILLUS, LOPOTRICHIC. 
B. syneyanus (Ehrb.) Migula=cyanogenes Fligge. Bacillus of blue milk. 
We have not found this species, but a culture was sent to us by Duckwall. Its 
characters, as determined by us, are as follows: 
Morphology.—Size. I.2u-2u x .54. Short chains; spores produced, but no 
capsules. Gram stain irregular. It possesses a tuft of flagella at one end. 
Gelatine colony.—A round, raised, smooth, entire colony, of a grayish color, 
-5 mm. in diameter, in three days. 
Gelatine stab.—A filiform, needle growth, and thin surface, which does not 
spread. After a few days the gelatine turns a dark color at the surface, but 
does not liquefy. 
Agar streak.—Luxuriant, spreading, thin, smooth growth, translucent, white, 
and showing a dark, smoky fluorescence. 
Fermentation tybes,—Dextrose and saccharose, rendered alkaline, without 
gas or closed arm growth, and no change in color. Lactose becomes very 
slightly acid, without gas or closed arm growth, and turns a deep blue-black. 
Boutllon.—A black sediment, and dark-colored turbidity, with a membranous 
pellicle. 
Milk.—Rendered slightly alkaline without curdling, but develops an odor, and 
after a few days becomes distinctly blue. 
Potato.—A very luxuriant, spreading, thick growth, translucent or opaque, 
brownish in color. ‘ 
Grows at both 20° and 37°. Aerobic. 
