174 STORRS AGRICULTURAL EXPERIMENT STATION. 
Gelatine colony.—A rapid liquefier with a membrane on the surface, which 
assumes a violet tinge in some cases. 
Gelatine stab,—A rapid liquefier, forming a cloudy liquid and a pellicle; 
violet in color. 
Agar streak.—A laxuriant growth, at first white and then violet; moist, 
wrinkled. 
Fermentation tubes.—Not given; probably no acid or gas. 
Boutllon.—A turbidity and a slight pellicle; the bouillon becomes violet. 
Milk,—The reaction is unchanged, or slightly acid. There is no curdling, 
but there forms a violet surface layer. 
Potato.—The original needle track is violet, but a dark brown growth covers 
the whole surface of the potato. Luxuriant. 
Ill. No pigment and no acid in dextrose or other sugars. 
B. lactis circulans f. and II, White circulating bacilli, These two varieties 
have not been found by us since their original isolation in 1895 as described 
previously. The two cultures, found at different times, differ from each other 
in the points indicated in brackets, which refer to No. II. Both of them showed 
the circulation which is characteristic of this type. Their characters are as 
follows: 
Morphology.—Size, 1.5u@x 6p. Forming chains. 
Gelatine colony.—A protruding bead or in a dry pit; then liquefying and 
showing a circulation in the liquid. 
Gelatine stab.—Liquefaction slow; a narrow funnel is formed with a dry pit 
above, and a rotating axis in the center of the funnel below. [Rotating axis 
absent. ] 
Agar streak.—Luxuriant, thick, yellowish. 
Bouillon.—A sediment, turbidity, and pellicle, but the liquid clears up after 
six weeks. 
Milk.—Slightly alkaline or unchanged in reaction. Digests without [with] 
curdling in 25 days, into a cloudy liquid with a sediment. 
Potato.—A rather scanty, thin, watery growth, white. [Reddish brown.] 
Potato sometimes discolored and sometimes not. 
Grows both at 20° and 37°— Aerobic. 
B. aerolactisn.s, A non-acid, gas-producing bacillus. This has been isolated 
several times from milk. In one case it was from a sample of old milk that 
developed a pleasant, fruity flavor. This culture produced in milk, however, a 
strong odor of decay. There are slight variations in the different cultures 
which we have isolated, but we regard them as identical. Some of the variations 
are indicated by brackets. 
Morphology.—Size, 1u-1.2u. x .4u-.8u. No chains have been found. Spores 
are produced, sometimes at the middle and sometimes at the end. There is 
frequently a capsule and the Gram stain is negative. [Capsule wanting.] 
