CLASSIFICATION OF DAIRY BACTERIA. 177 
Agar streak.—Luxuriant, umbonate, smooth, gray, moist, iridescent. 
Fermentation tubes.—Acid, gas, and closed arm growth in dextrose. Closed 
arm growth in other bouillons but no acid nor gas. 
Bouillon. — A granular sediment, a decided turbidity, and a flocculent 
pellicle. 
Milk.—Rendered alkaline, digested, curdled, and subsequently digested into 
a gelatinous mass at both 20° and 37°, with an unpleasant odor. Later the 
odor is of old cheese. 
Potato.—A filiform, capitate, smooth, gray growth, not very luxuriant, with 
potato discolored. 
Grows abundantly at 20° and 37°. Aerobic. Found in Camembert cheese. 
Variety A.. . 
Morphology.—Size, 2u-3 x .5u-.74. No chains, no spores, a very evident 
capsule and Gram stain positive. 
Gelatine colony.—A very slow liquefier, not characteristic. 
Gelatine stab.—An arborescent needle growth, with crateriform liquefaction, 
beginning in one day. 
Agar streak.—Filiform, flat, contoured, transparent, gray, luxuriant, moist. 
Fermentation tubes.—No gas, acidity, nor closed arm growth in any bouillon, 
Saccharose is rendered acid. No other effect in any sugar bouillon. 
Boutllon.—A slight sediment is produced, but no other effect. 
Milk.—No action on milk. 
Potato.—Spreading, thin, smooth, translucent, luxuriant, gray, moist. Potato 
discolored. 
Grows at 20° and moderately at 37°. Aerobic. 
B. lactis plicatus n. s. A spore bearing, liquefying bacillus with a folded 
scum. Found in Camembert cheese. 
Morphology.—Size, 24x .8u-1.2u. No chains, central spores produced, Gram 
stain positive, no capsules. 
Gelatine colony.—Rapidly liquefying into a non-characteristic cloudy pit. 
Gelatine stab.—At first arborescent; liquefies in one day, stratiform; about 
one third of the gelatine liquefies in ten days, with a white folded scum. 
Agar streak.—Nodose, capitate, rugose, opaque, white, luxuriant, moist and 
wrinkled. 
Fermentation tubes. — Dextrose is rendered acid; no gas and no closed arm 
growth in any sugar bouillon. 
Bouillon.—A granular sediment, a turbidity, and a pellicle forms, which sub- 
sequently sinks. 
Milk.—Becomes alkaline and curdles at 37°, not at 20°. Is subsequently 
digested. 
Potato.—Luxuriant, diffused, thin, smooth, of an orange-white color. Potato 
is discolored. 
Grows at 20° and 37°, but better at 37°. Aerobic. 
