CLASSIFICATION OF DAIRY BACTERIA, 179 
Gelatine stab.—A needle growth, liquefying in one day, infundibuliform; com- 
plete in eight days. 
Agar streak.—Scanty, linear, thin, smooth, opaléscent, gray, moist. 
Fermentation tubes.—All three sugar bouillons show acidity and growth in 
the closed arm,, but no gas.. 
Bouillon.—An amorphous sediment and a slight turbidity, but no pellicle. 
Milk,—Becomes acid, and curdles at 20° and 37°, and subsequently digests, 
giving a clear whey, with a vinous odor. 
Potato.—Scanty, linear, thin, smooth. Potato not discolored. 
Grows at 20° and 37”, but better at 20°. Facultative anaerobic. 
Close to this belong the butyric acid organisms of Prazmowski, Hueppe and 
Botkin. We have not studied them. 
B. lactis Pruchiin. s. A slimy milk, peritrichic bacillus. This peculiar bac- 
terium was sent from Geneva. Its remarkable involution forms, and its other 
unique characters clearly distinguish it, although it was lost before we had quite 
completed our work upon it. 
Morphology.—A spore-producing, peritrichic bacillus, with no capsule. It 
does not accept the Gram stain. Involution forms, curved, club shaped, and 
showing other irregularities, are common. 
Gelatine colony.—A rapidly liquefying pit, not characteristic. 
Gelatine stab.—Liquefies in one day, stratiform, with a turbid liquid, and a 
reddish-yellow sediment. " - 
Agar streak.—Round, flat, smooth, opaque, white, luxuriant and viscous. 
No fluorescence is seen, although milk is turned green. 
Fermentation tubes.—Dextrose bouillon is rendered acid. No gas and no 
closed arm growth in any bouillon. 
Bouillon.—A viscous sediment, a turbidity, and a flocculent pellicle. 
< Milk.—I|s rendered acid, is curdled and digested at both 20° and 37°, witha 
slight yellowish color. Later it becomes quite yellow and slimy. 
Potato.—Spreading, thin, smooth, brownish, luxuriant. Potato discolored. 
Grows at both 20° and 37° Anaerobic. 
B. lactis fungiformis n.s. A white, rhizoid, spore-producing bacillus. This 
has been found in fresh milk and stable dust several times. The different cul- 
tures show slight variations, indicated within brackets. 
Morphology.—Size, 34-3.5¢X 1.3u. No chains. Spores are developed and 
an evident capsule. The Gram stain is positive. 
Gelatine colony.—The colony throws out fibers like a mold, but after two days 
this character disappears, the colonies disintegrating into a liquefying pit. On 
Litmus gelatine this character is not evident. 
Gelatine stab. Liquefaction begins in two days, but never becomes complete. 
Infundibuliform. 
Agar streak, Filiform, raised, grumose, translucent, porcelain white, dull 
[wrinkled]. 
