II 



the purple colour is developed the solution is quite 

 useless. 



Solution B. Eosin . i part. 



Water . . i,ooo parts. 



For staining, these stock solutions are diluted 

 one in twenty, respectively, with water, i.e., five 

 parts of the stock are made up to one hundred 

 parts with water. 



To Stain. — Mix equal parts (say one c.c.) of 

 each solution and pour on the slides. 



Leave the stain on the slides any time from 

 ten minutes to half-an-hour or longer. Wash off 

 the excess of stain with water, and allow them to 

 drain or dry them with blotting paper, but do 

 not dry them by heating over a flame. The red 

 corpuscles may have a bluish tinge. This can be 

 got rid of if desired by washing in water or very 

 rapidly in equal quantities of spirit and water. 



Placed under the microscope, while still wet, 

 the blood platelets should appear as ruby red 

 granular masses, if they are bluish the film should 

 be replaced in the staining solution. 



Slides may be decolourized to any required 

 extent by soaking in water, in fact, if left long 

 enough the stain is entirely washed out. Such a 

 specimen can, however, easily be stained a second 

 time. 



The exact position and relations of pigment 

 are best seen in specimens lightly stained, as deep 

 RoMANOWSKY staining may completely obscure 

 pigment. 



Method 2. Leishman's stain : — 



Leishman's stain consists of the product of 

 inter-action of the eosin and the methylene blue 

 of the first method. , 



