12 



Make the following solution, viz. : — 



Leishman's stain . . 0-15 grammes. 

 Methyl alcohol . ., . iioo;o c.c. 



Place sufficient solution on the slide to cover 

 the film, and allow it to stand for about half-a- 

 minute. Add about twice as much water. Mix 

 by moving the slide to and fro, or stir gently with 

 a glass rod. Allow it to stain five minutes or 

 longer. 



The stain is also sold in the form of ' solqids ' 

 (by Burroughs, Wellcome & Co.), each ' soloid ' = 

 o"oi5 grammes. If it is impossible to procure 

 methyl alcohol (pure), dissolve the ' soloid ' in 

 methylated spirit (ten c.c.) and proceed as above. 

 The results got with methylated spirit are per- 

 fectly satisfactory for diagnostic purposes. There 

 is no preliminary fixing. 



TuLLocH recommends making a saturated solution of the 

 stain in twenty-five c c. of metfiylated spirit, to which two 

 drops of a ten per cent, solution of potassium bicarbonate have 

 been added. 



After some minutes the slide will be stained. 

 The same red scum and precipitate are seen as 

 in Method i, and are of the same significance. 

 The slide should, when stained, be washed in water 

 and allowed to remain in this for a minute or so. 

 This intensifies the Romanowsky staining and 

 removes the remains of the deposit. The red cells 

 also by this process are changed from greenish to 

 faint pink. 



To obtain the most brilliant results with 

 these stains is perfectly easy, and no one who has 

 used them will, except for special reasons, use 

 any others at present in use. 



'r''M 



