31 



To Stain Flagellated Bodies. 



1. When flagellation is observed the cover- 

 glass is forcibly ' smeared ' off, and slide and 

 coverglass are then fixed and stained with Roman- 



OWSKY. 



2. A number of rather thick drops of blood 

 are placed on a series of slides. These are inverted 

 over rectangular holes cut in blotting paper, 

 moistened with water, and spread on a sheet of 

 glass. A series of moist chambers is thus made. 

 A dozen or more films are made, and each one is 

 removed at intervals of five minutes, dried 

 (spreading out somewhat if necessary), fixed, and 

 stained with Romanowsky ; or dry the thick 

 film, decolourize with water, stain with Roman- 

 owsky (without fixing), as in Professor Ross' 

 method of making thick films. 



To Determine the Species of Parasite 

 Present 



Three forms are recognized — simple tertian, 

 malignant tertian, and quartan. The malignant 

 tertian can, as we shall see, produce a quotidian 

 temperature with only a single generation of 

 parasites. Whether or no there is a true quo- 

 tidian parasite, one or more, is extremely doubtful. 



1. Minute rings, one-sixth to one-seventh 

 the diameter of a red cell, showing the signet 

 ring shape, are characteristic of malignant tertian 



(Fig. 4)- 



2. Large rings. — If, when the temperature 



of the patient is still high, the rings are of con- 

 siderable size, one-fourth to one-third of the red 



