43 



Chapter V 



THE PARASITE IN THE TISSUES 



Tissues may be readily examined for the 

 presence of parasites or pigment in the following 

 way : — Place a minute portion of the -tissue on a 

 slide, and with the end of another slide spread it 

 out as evenly and thinly as possible. Dry, fix, 

 and stain in the same way as a blood film. Para- 

 sites, if present, are in this way much more easily 

 .and clearly seen than in sections. Spleen pulp, 

 bone marrow, kidney, liver, etc., give beautiful 

 results, and in the same way any secretion or fluid 

 can be examined. For certain tissues, e.g., bone 

 marrow, it is advisable to fix in — 



Absplute alcohol, i part, 



Ether . . .2 parts, 

 in order to dissolve out the fat present. 



To Prepare Tissues 



In preparing tissues for examination :- - 



1. Use as small pieces as possible, i.e., at 

 most five mm. thick. 



2. Use plenty of the fixing and hardening 

 fluid because the tissues contain much water, and 

 if the fixing fluid becomes dilute it acts as a 

 macerating agent. 



3. See that the separate pieces do not cohere 

 to one another. Place some cotton wool on the 

 bottom of the vessel. 



