52 



B. — Leishman, before staining, places some fresh blood 

 serum on the section for five minutes. Stain with 

 Leishman's stain, using two or three lots of fresh 

 stain for one or two hours. Differentiate alter- 

 nate!}' with two solutions, i in 1,500 acetic acid 

 to remove excess of blue, and i in 7, coo caustic 

 soda to remove excess of eosin. Watch effect 

 with a low power. Dehydrate rapidly with 

 alcohol. Xylol. Balsam. 



