99 



Place the whole, after labelling, in a dark cup- 

 board or other convenient place (incubator). 



At the end of the first day or so, the males 

 will be found dead upon the piece of paper, and 

 can be removed. On the second night after 

 hatching, most of the insects will feed, and the jar 

 is ready for use. 



2. Place the inverted (chutney) jar, prepared 

 with cardboard as above, over a bottle in which 

 Anophelines,ca.ughtm a village or elsewhere, have 

 been placed. Remove the cotton plug and shake 

 the bottle gently to drive the insects out. Replace 

 jar upon the prepared stopper. Place in a dark 

 spot. Next morning remove the stopper and re- 

 move any dead mosquitoes and ova by taking out 

 the piece of paper. 



On the second night after the mosquitoes 

 have been collected, the bottle is ready for feeding 

 experiments. On the third day, generally, the 

 mosquitoes have no longer any blood remaining 

 in the mid- gut, and are ready for dissection. 



The glands of any mosquitoes that may die 

 before this may of course be dissected, if desired, 

 on the chance of finding sporozoits. 



In the use of village-caught Anopheltnes, it must 

 be borne in mind that any subject upon which 

 they are fed is liable to a fresh infection. In the 

 case of natives (who sleep without hesitation in 

 any village), the employment of village-caught 

 mosquitoes cannot, however, be very prejudicial. 



The advantages of the above way of keeping 

 mosquitoes are : — 



1. The mosquitoes will keep alive longer 

 than in ariy other way known to us. 



2. The immense convenience in feeding. 



