IO-2 



Repeat the process every night, allowing the 

 mosquitoes to feed by preference on the same case 

 throughout. Where it is uncertain wliich of several 

 cases may or may not have mature sexual forms 

 in the blood, a bottle may be fed on alternate 

 nights on the different cases. 



Add clean water and a fresh piece of paper 

 each time the bottle is used. 



To Prepare Fed Mosquitoes for Dissection 



After having fed the mosquitoes in a bottle 

 for a certain number of days, place it apart from 

 others, and allow it to remain undisturbed 

 (merely changing the water, etc.) for several 

 days. 



Ascertain each day whether the mosquitoes 

 have completely got rid of the blood in the mid- 

 gut. When they are quite free from any dark 

 colouration of the ventral aspect of the abdomen 

 they are ready for dissection. 



N.B. — If chloroform, and especi.illy if tobacco smoke is used 

 to icill the mosquito, it is essential to well wash the jars before 

 again keeping mosquitoes in them. 



To Feed Mosquitoes on Birds, etc. 



I. Prepare a framework of wood and book- 

 binder's cardboard. Cover two sides with card- 

 board. Cover one end with netting drawn tight, 

 and to the other attach a ' sleeve ' of netting. 

 Catch or breed out a number of Culex (e.g., Culex 

 fattgans), and place in the frame. Keep the frame 

 in a dark place, and place a saucer of water 

 in it. 



