Ill 



Note that at the point of breaking up, one can 

 generally make out large stellate cells (tracheal 

 cells). (Fig. 31.) 



7. Observe in some preparations, large oval 

 cells of brownish colour lying upon the outer 

 surface of the stomach. Note that they are rather 

 opaque, and contain a certain amount of diffuse 

 yellowish pigment. They are so called pericardial 

 cells (see Fig. 32). 



8. Observe, in most preparations, one or 

 more large clear cells with a small nucleus and 

 filled with oil globules (cells of the fat body) 

 (Fig 32.) These lie upon the stomach and, in 

 common with the last named cells, are accidental 

 in this situation. 



The Examination of the Mid-Gut for the 



Zygote or Oocyst Stage of the 



Malarial Parasite 



[The examination of the stomach Mood for flagellating and 

 the motile or vermicide forms is deferred to a later Chapter). 



Obtain a number of Anophelines (not M. vossii) 

 from some native quarter (see p. 92), or better, 

 those specially fed. Keep these alive for two 

 or three days until no blood remains in the 

 mid-gut (for methods of keeping alive, see p. 95). 



Prepare the mid- gut as described above. A 

 considerable number may prove negative, but a 

 variable percentage will be positive. Examine 

 with one-twelfth inch. 



Carefully note the presence of small collections 

 of pigment of the nature of malarial pigment. 

 By careful focussing, the younger forms may be 



