113 



3- In still more developed forms, the sporo- 

 blasts are seen to be surrounded by a radiating 

 arrangement of young sporozoits or blasts (Figs. 9 

 and 31). 



4. Fully developed forms are large cysts 

 packed with many hundreds of fine sickle-shaped 

 bodies, and, if they are ruptured, these latter escape 

 into the surrounding fluid, and are readily dis- 

 tinguished as sporozoits (Fig. 37). 



Fig. 33. (Left to Right) Zygotes of Malignant Tertian, 

 Simple Tertian, Quartan, and Proteosoma 



To Make Permanent Preparations of 

 Zygotes 



Method I. — In case of a specimen shewing 

 zygotes, place a large drop of two per cent, formalin 

 on one side of the coverglass, and draw this 

 through b}? filter paper placed on the other side. 

 Repeat several times. Remove excess of the 

 formalin with filter paper. Ring edges of cover- 

 glass with black varnish or Canada balsam. 



The zygotes will retain their appearance as 

 seen in the fresh specimen. 



Method 2. — Run formalin through as in 



Method I. When an excess of fluid is present, 



slide off the coverglass. The flattened mid-gut 



will probably remain attached to the coverglass. 



I 



