ii6 



but if found necessary they can be teased out further 

 and placed in fresh drops of salt solution. 



g. At all stages of the dissection make sure 

 that the glands are really present and that they 

 have not floated to the side or stuck to the 

 needles. 



lo. By this certain and rapid method, as 

 many as one hundred glands may be dissected 

 out, put under a coverglass, and examined micro- 

 scopically in a day's work. 



3otf«/ tS^^-r,/.' 



rf.cf 



Fig. 35. The Salivary Glands of one side 



Method 2. — Consists in isolating, by a series 

 of cuts, the anterior ventral portion of the thorax 

 in which the glands lie. 



1. Make a cut obliquely in an antero- 

 posterior direction, so as to sever the main mass 

 of thoracic muscle. 



2. Make a cut at right angles to this, passing 

 just behind the attachment of the first pair of 

 legs. 



3. Cut through the neck. 



4. The glands lie in the portion thus iso- 

 lated. Considerable teasing out is still required 

 to isolate them from the surrounding tissues. 



