iig 



Examination of the Sporozoit Form of the 

 Malarial Parasite 



Obtain a number of Anophelines (not M. rossii) 

 from a native quarter (five per cent, to twenty per 

 cent, or more have sporozoits in the glands), or 

 Anophelines fed for twelve days or more at a tem- 

 perature of 80° F. Prepare specimens of the glands, 

 as described above. Having placed one or more 

 lobes under a low power, press with the point of 

 a needle on the coverglass, so that the gland is 

 ruptured, and the secretion poured out as droplets 

 into the surrounding fluid. 



Examine with one-sixth inch. If sporozoits 

 are present they are generally very numerous, and 

 large numbers of fine, very distinct curved rods, 

 will be easily seen with this power, lying through- 

 out the fluid around the gland and packed in large 

 numbers in the substance of the gland. Finally, 

 examine with one-twelfth inch (Fig. 37). 



The sporozoits have a mean length of 14 m, 

 and vary between 10 /u and 20 /j., and are 1-2 /^ in 

 width. 



■ Examination of Motion of Sporozoits 



Dissect out the glands and, when isolated 

 cleanly, transfer to a drop of human serum, 

 previously got ready by allowing blood to clot in 

 a small tube. Three kinds of motion may be 

 observed : — 



1. Formation of curves. 



2. Formation of ring-formed contractions. 



3. Locomotion. Forward motion. 

 Penetration of Red Cell by Sporozoits. — This 



has not been seen in case of sporozoits of the 



