128 



of corrosive sublimate (saturated whilst hot and 

 allowed to cool). 



As this is a watery solution, practically no 

 shrinking occurs. It, unfortunately, cannot be 

 used for the whole mosquito, as watery solutions 

 will not penetrate the tissues. 



Allow to remain in corrosive solution ten 

 minutes. Place in large volume of water for 

 twelve hours, and in weak watery iodine solution 

 (sherry colour) for one hour. Pass through 30 per 

 cent., 50 per cent., 75 per cent., to absolute 

 alcohol. Allow to remain in absolute alcohol 

 fifteen minutes. Transfer to xylol, three mirlutes ; 

 paraffin two minutes. Cut in ribbons and treat 

 as already described. 



To Cut Sections of the Salivary Glands 



Embed only the head and front half of the 

 thorax. Cut sections as near as possible hori- 

 zontally, this will give sections in the length of 

 the glands, and both sides at the same time. 



Stain with various stains : Heidenhain's hae- 

 matoxylin, gold chloride, etc. Much work remains 

 to be done upon the structure and nature of the 

 glands, and the method by which the sporozoits 

 reach the salivary cells. 



LITERATURE : 



Gr.issi. The Studies of a Zoologist (in German or Italian). 



