159 



and observe that in all, Anopheline, as well as 

 Culicine larvae, may abound. 



Note that in waters covered with certain 

 species of Lemna, Anophelines are rarely found. 



(v) Place any larvae (nearly adult specimens 

 if possible,) and nymphae in collecting tubes with 

 a note as to where they have been obtained. 

 If it is necessary to cork the tubes, some air 

 space should be left and the corks loosened as 

 often as possible. Larvae, as a rule, survive 

 carriage in small collecting tubes better than they 

 do in bottles or larger vessels. They may be 

 carried long distances, e.g., in a train or on horse- 

 back, provided that occasionally the tube is un- 

 corked and they are allowed breathing time. Larvae 

 survive rough treatment better than pupae, and 

 when apparently dead, may often be revived by 

 floating out on the surface of the water. Examine 

 the larvae (Chap. IX), and roughly divide them 

 into as many groups as possible, observing the 

 main characters of each. 



(vi) Place each variety in small bottles, over 

 the neck of which a piece of mosquito netting 

 must be tied as soon as the larvae have turned 

 into nymphae. 



When the adult insect has hatched out, note 

 its attitude and any other special features. 



To Kill Mosquitoes 



I. A mosquito that has just hatched out from 

 the nympha should not be killed for some hours 

 until its exoskeleton has hardened. If it is killed 

 immediately, the wings on drying will shrivel, and 

 possibly the whole insect become distorted. 



