268 



(i) The percentage of infected children in 

 each group. 



(ii) The degree of infection of the adults. 



(iii) Roughly estimate the number of Ano- 

 pheles present, whether swarming, abundant, scanty, 

 or impossible to detect by search. In the latter 

 case make several ' test pools.' 



(iv) Determine the species present and the 

 relative numbers of each. 



(v) Determine the sporozoit rate for each 

 species. 



(vi) Carefully map all breeding-places, noting 

 wha!t larvae are found. 



4. Capture as many Anqphelines as possible in 

 the European houses, especially in the morning, 

 and by looking within the nets. Determine the 

 species, sporozoit rate, and from where probably 

 derived. Examine the ovaries andspermatheca,and 

 note; whether freshly hatched or impregnated 

 females are chiefly found. Note the presence or 

 absetice of males. 



In investigating the malaria of any such 

 settlement, native and European, continue the 

 observations if possible throughout the year. 

 Make observations on — 



1. Seasonal variations in the endemic index 

 (percentage of infected children). 



2. Seasonal variations in the number of 

 cases among Europeans. 



3. Prevalence of any particular species of 

 Anophelines at any time of the year. 



4. Distance of flight of Anophelines from 

 breeding- grounds, etc. 



5. Sporozoit rate of Anophelines at different 

 times of the year. 



