271 



.Chapter XXIV 



CLINICAL STUDY OF MALARIA 



Enumeration of Red Cells 



In blood counting, much practice only can 

 give accurate results ; inaccurate results are mis- 

 leading and useless. For comparative purposes, 

 counts should be made always at the same time, 

 if possible, to obviate the effect of food, etc. 



For diluting the blood, o"g per cent, salt 

 solution may be used, or preferably,ToisoN's fluid, 

 which has the following formula : — 

 HjO . 160 c.c. 



Glycerin . 30 c.c. 



Sodium sulphate 8 grammes 

 Sodium chloride i gramme 

 Methyl, violet (or other stain sufficient to 

 colour the nuclei of the leucocytes) 

 The diluting fluid must always be poured into a 

 watch glass, and it should not be sucked up out of 

 the stock solution. 



No pressure must be used to make the blood 

 drop exude from the finger. 



Blood is then sucked up to the mark i on the 

 pipette, and the end of the pipette carefully wiped 

 before plunging it into the Toison's fluid. The 

 ToisoN is sucked up to the mark loi exactly. The 

 pipette is then rotated between finger and thumb, 



