311 



allow the serum to separate. Observe whether 

 the serum is yellow (cholaemia) or reddish 

 (haemoglobinaemia), using the spectroscope if 

 necessary. 



4. To some of patient's serum add normal 

 blood. Observe whether there is any haemolysis 

 (using a haemocytometer if necessary). 



5. Determine tonicity of patient's blood. 

 Rate of coagulation approximately by placing 

 several drops on a glass slide. 



6. Count the red and white cells. The red 

 cells are, as a rule, quite normal in shape. 



7. Determine the amount of haemoglobin. 



8. Make films every two hours if possible 

 (fis early as possible), noting accurately the time 

 and temperature at which the films are made. 



9. Examine films for parasites ; if these are 

 absent, search carefully several large films for 

 pigmented leucocytes, as these, as also in ordinary 

 malaria, may require long search. 



10. Make careful differential counts of the 

 leucocytes, especially when the temperature is 

 falling, as it is then that the mononuclear increase 

 is most marked. When the temperature is raised 

 (e.g., 103° to 105°) the polynuclears may reach 

 ninety per cent. 



11. Observe presence of normoblasts, megalo- 

 blasts, various abnormal staining reactions, e.g., 

 poh'chromatophilia of the red cell, especially dur- 

 ing recovery. 



12. Make careful blood counts immediately 

 before and after administering quinine when 

 no haemoglobinuria results. According to Panse* 

 there may result a blood destruction due to the 



*Panse. Zeiuchrift fur Hygiene^ 1903) s. 1. 



