359 

 TRYPANOSOMATA OF MAMMALS 



Examine the blood or the oedema fluid, if present, of 

 camels, cattle, horses, dogs, etc , especially those showing 

 emaciation, oedema of any part, watery discharge from the eye, 

 skin eruptions, etc, Apparently healthy animals may not in- 

 frequently harbour trypanosomes, e.g., calves in India (Madras), 

 the big game in Africa, etc. Compare carefully the trypano- 

 somes found. 



Fig. 80. T. lewisi. — (i) Dividing form ivith two blepharoplasts ; 



(2) Adult form ; (3) Young forms resulting from 



division {fresh preparation) 



Trypanosomes are bodies easily detected in 

 fresh blood with a one-sixth or one-seventh lens. 

 They are actively motile, and may be seen dis- 

 placing the red cells by their motions. As they 

 come to rest the undulating membrane and flagel- 

 lum are visible. They are bodies about twenty 

 M long. In stained specimens (Romanowsky) an 

 oval nucleus lies about the middle of its length, 

 and near the blunt posterior end a small stained 

 particle is clearly seen, the centrosome, or rather 

 blepharoplast. From this, the fiagellum starts, 

 'and can be seen as a distinct wavy thick red 

 line extending the whole length of the organism 

 and continued beyond as the long (anterior) 

 free fiagellum. The portion (unstained) between 

 this external wavy margin and the blue stained 

 body of the organism is the undulating membrane 

 (Fig. 80). 



