390 



5- Three well-marked pre-anal papillae and 

 two unequal post-anal papillae. Two slender 

 unequal spicules. 



F. megalhaed. — Adult males and females in 

 left ventricle. 



? I. Length, one hundred and fifty-five mm. 

 by 0"7 mm. 



2. Club-shaped oral end. 



3. Swollen oesophagus well marked. 



4. Mouth simple. 



5. Cuticle, fine striations. 



$ I. Length, eighty-three mm. by 0-4 mm. 

 2. Four pairs pre-anal,four post- anal papillae, 

 and two spicules. 



To Examine Blood for Filaria Embryos 



The technique varies somewhat according 

 to what end the observer has in view. 



1. To facilitate detection, it is well, as 

 Manson advises, to make thick films of blood. 

 Dry. Then wash out the haemoglobin with water 

 or one-third per cent, acetic acid, and stain with 

 haematin, or gentian violet, or fuchsin. 



For the latter stains, a few drops of a satu- 

 rated alcoholic solution of the dye are added to 

 half a watch-glassful of water. 



Search the slides stained (or fresh) with a 

 half-inch lens. 



2. (For studying the minute structure of 

 the embryos, the above method is not advisable). 

 Make a film in the ordinary way. Fix in alcohol 

 and stain with haematein. The 'spots' and 

 granules of the embryo are most beautifully 

 shewn. 



