XXUl 



4. Safvanin. — Make a saturated solution in two per cent, 

 anilm water. Heat to sixty per cent. C. Filter hot. Stain 

 sections over night. DifEerentiate very carefully with one per 



■ cent, hydrochloric alcohol. Safranin is an excellent nuclear 

 stain. 



5. Ehrlich's Triacid. Stain for five minutes. 



6. Ehrlich's Haematoxylin-Eosin. — Haematoxylin, five 

 grammes ; acetic acid, twenty grammes; alcohol, one hundred 

 grammes ; glycerine, one hundred grammes ; water, one 

 hundred grammes. Allow this to ripen for a month in the 

 sun, then add eosin to the extent of one per cent. ; stain for 

 twenty-four hours. The solution is best got ready-made. 



Solubility of Stains 



10 c.c. of saturated alcoholic methylene blue contains 

 o'o68 grammes, of the stain. 



10 c.c. of saturated aqueous methylene blue contains 

 o'664. grammes of the stain. 



10 c.c. of saturated alcoholic gentian violet contains 

 0'4i|2. grammes of the stain. 



10 c.c. of saturated aqueous gentian violet contains o'lyj 

 grammes of the stain. 



ID c.c. of saturated alcoholic fuchsin (basic) contains 

 o'2g2 grammes of the stain. 



10 c.c. of saturated aqueous fuchsin (basic) contains o'o56 

 grammes of the stain. (Hewlett). 



Lojfier's Methylene Blue. — 30 c.c. saturated alcoholic 

 methylene blue. 



100 c.c. of o'l per cent, caustic potash. 



Ordinary Methylene Blue. — A saturated alcoholic solution 

 is used as the stock, and a few drops added to a watch-glass- 

 ful of water, according to strength required ; or ten per cent, 

 solution is a convenient strength. 



Borax Methylene Blue (Sahli's). — Saturated aqueous solu- 

 tion of methylene blue, twenty-four parts ; borax five per cent, 

 solution, sixteen parts ; water, forty parts. 



The times necessary for staining are best judged by the 

 appearance of the films or tissues. 



Tissue Stains 



I. Haematein (p. 50). — Stain for about five minutes, 

 according to the ripeness of the solution. Sections do not 

 readily overstain. Decolourize, if necessary, with one per cent. 



