XXIV 



alum solution. Counterstain, if required, with a weak, watery 

 solution of eosin, one-half to one per cent. ; or the sections 

 may be stained first with a strong eosin solution, five to one 

 hundred per cent., for five to twenty minutes. Combination- 

 of eosin and methylene blue can be used in a similar way. 



2. Alum Carmine. — Carmine, two grammes ; alum, five 

 ■grammes ; water, one hundred c.c. Boil together for one 

 hour. Filter. Does not ovt-rstain. 



3. Ehrlich Biondi. — Saturated aqueous solution of acid 

 fuchsin, four parts ; orange g., seven parts ; methylene green, 

 eight parts. Dilute fifty to one hundred times before using. 

 Stain for twenty-four hours. Wash in alcohol. The sections 

 may, before staining, be treated with acetic acid (two parts in 

 one thousand of water) for a few hours. 



Iron Reaction (Haemosiderin) in Malarial Tissues 



1. Fix in alcohol. 



2. Two per cent, aqueous solution of potassium ferro- 

 cyanide, five to twenty minutes. 



3. Acid alcohol "(HCl, one part, seventy i^ercent. alcohol, 

 one hundred parts), five to ten minutes. 



4. Wash in water. 



5. Counterstain with alum carmine. 



Staining of Amoeba Coli in Tissues 

 (Mallory and Wright) 



1. Fix in alcohol. 



2. Saturated aqueous solutions of thionin, three to five 

 minutes. 



3. Two per cent, solution of oxalic acid, one-half to one 

 minute. 



4. Wash in water. 



5. Dehydrate in alcohol. 



6. Clean in oleum origanum cretici. 



7. Wash in xyol. 



8. Balsam. 



Nuclei of the amoebae are brownish red, the nuclei of the 

 mastzellen ' are blue. 



Weights and Measures, etc 

 I. Conversion from one temperature scale to another — 

 9 z= 5 = F-32 

 5 4 9 



