ASIATIC CHOLERA. " 109 



tion of urine, which is albuminous. The animals recover 

 very slowly. In this connection Bouchard remarks that 

 in 1884 he obtained by the intravenous injection of the 

 urine of a cholera patient in rabbits muscular tremor, cyan- 

 osis, albuminuria, and diarrhoea, but that he has never suc- 

 ceeded in inducing these symptoms with the cholera vibrio. 



Petri finds that the comma bacillus produces in solu- 

 tions of peptone large amounts of tyrosin and leucin, a 

 small quantity of indol, fatty acids, poisonous bases, and a 

 poisonous proteid. The proteid resembles peptone in its 

 behavior toward heat and chemical reagents, and is desig- 

 nated by Petri as " toxopeptone." It is not precipitated 

 by heat or concentrated nitric acid, nor by potassium ferro- 

 cyanide and acetic acid, nor by ammonium sulphate added 

 to saturation. With sodium phospho-tungstate it gives a 

 precipitate which clears up on the application of heat. The 

 precipitate with tannic acid is insoluble in an excess of the 

 precipitant. It gives the biuret reaction perfectly, but 

 responds to Millon's test but feebly. 



In quantities of 0.36 of a gramme per kilogramme and 

 more it is fatal to guinea-pigs within eighteen hours. It 

 produces muscular tremor and paralysis. Post-mortem 

 shows an effiision into the peritoneal cavity, marked injec- 

 tion of the bloodvessels of the intestines, and isolated 

 hemorrhagic spots. 



This proteid is not rendered inert by a temperature of 

 100°. Petri does not claim that he has obtained a chemi- 

 cally pure body, but supposes that it is contaminated with 

 more or less unchanged peptone. 



ScHOLL has studied the chemical products of the cholera 

 bacillus when grown under anaerobic conditions. Fresh 

 eggs were sterilized and inoculated in the usual way. The 

 eggs, after being kept for eighteen days at 36°, were opened. 

 The contents smelled intensely of hydrogen sulphide, but 

 not of ammes. The albumin was completely fluid, while 

 the yolk was more solid and of a dark color. 



Five cc. of the fluid contents were injected into the 

 abdomen of a guinea-pig. Soon the posterior extremi- 

 ties were paralyzed, and after ten minutes the paralysis 



6 



